Measurement of IgD remains one of the mainstays of diagnosis of HIDS. To this end, we have developed a sensitive enzyme-linked-immunosorbent assay (ELISA). The serum IgD is expressed as IU/ml. For comparison: 1 IU/ml equals 1.412 mg/l. The lower limit of detection is 1.4 mg/l.
For diagnosis of HIDS the patient should have a typical clinical history and a high serum IgD (> 100 IU/ml) measured on 2 separate occasions, one month apart.
In the Laboratory of Clinical Chemistry in the Radboud University Medical Center Nijmegen IgD is measured on a routine basis. Physicians can submit samples to this laboratory after consultation with Dr. Ina Klasen (E-mail: I.Klasen@abti.umcn.nl).
Samples
Samples should be submitted as serum and we regard 5 cc as the minimum. Please write the name and date of birth of the patient as well as the sender on the tube. Preferably, mailing of samples should be done on a Monday or Tuesday. Please only send samples after consultation with Dr. Ina Klasen.
Method
Microtiter plates are directly coated for the titration of human IgD with rabbit anti-human IgD. After overnight incubation at 4 C, plates are washed, serum is added to the plates, and plates are incubated again overnight. A standard human serum, with a known concentration of IgD, is used as a reference. The plates are washed and mouse monoclonal anti-human IgD-Fc is added and after a washing step, HRP rabbit anti mouse anti IgD is added. After a washing step, IgD is measured by incubation with orthophenylenediamine and the plates are read with a Titertek Multiskan ELISA reader.
Last update: February 11, 2006
